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biotinylated goat anti guinea pig secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat anti guinea pig secondary antibody
    Biotinylated Goat Anti Guinea Pig Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+guinea+pig+secondary+antibody/Biotinylated+Goat+Anti-Guinea+Pig+IgG+Antibody/pmc13043728-280-6-20
    Average 95 stars, based on 973 article reviews
    biotinylated goat anti guinea pig secondary antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Parvalbumin interneurons in the hippocampal formation of individuals with Alzheimer’s disease: a neuropathological study of abnormal phosphorylated tau in neurons
    Article Snippet: The next day, the sections were incubated for 2 h at room temperature with a mixture of Alexa fluor-conjugated secondary antibodies: goat-anti mouse 594 and goat-anti rabbit 488 (1:1000, A11005 and A11008, respectively; Molecular Probes). .. Alternatively, sections were incubated with biotinylated goat anti-guinea pig secondary antibody (1:200; BA-7000; Vector Laboratories), followed by a mixture of Alexa fluor 594 anti-rabbit (1:1000, A11012, Molecular Probes) and streptavidin coupled to Alexa fluor 488 (1:1000; S32354; Molecular Probes). .. After rinsing in PB, the sections were treated with Autofluorescence Eliminator Reagent (2160, Chemicon) to reduce lipofucsin-like autofluorescence, as autofluorescence is a potential confounding factor , due to the accumulation of lipofuscin with age ( ).

    Article Title: Modulation of striatal glutamatergic, dopaminergic and cholinergic neurotransmission pathways concomitant with motor disturbance in rats with kaolin-induced hydrocephalus
    Article Snippet: Sections were then incubated with guinea pig anti-substance P (ab10353; Abcam) antibody for 18 h at 4 °C. .. After several rinses in PBS, sections were incubated with biotinylated goat anti-guinea pig secondary antibody (BA-7000; Vector Laboratories) for 1 h, and then with avidin–biotin-complex alkaline phosphatase conjugate reagent (AK-5000; Vector Laboratories) for another hour at room temperature. ..

    Article Title: Capsazepine decreases corneal pain syndrome in severe dry eye disease
    Article Snippet: Then, brain sections were incubated for 1 h in a blocking solution of 0.1M PBS containing 3% normal goat serum and 0.1% triton X-100, followed by incubation with the primary antibody polyclonal guinea pig anti-c-Fos (Synaptic Systems, ref 226 004, 1:1000) at 4 °C for 72 h. All steps following incubation with the primary antibody were performed at room temperature (RT). .. Next, brain sections were rinsed in 0.1M PBS-T and incubated with biotinylated goat anti-guinea pig secondary antibody (Vector, ref BA-7000-1.5, 1:500) for 1 h at RT. .. Samples were washed in PBS and incubated with VECTASTAIN Elite ABC HRP (PK-6100, Vector, A reagent 1:250 and B reagent 1:250) in PBS for 1 h at RT.

    Article Title: Effects of propofol on sleep architecture and sleep-wake systems in rats.
    Article Snippet: Previous studies have shown that the synchronization of electroencephalogram (EEG) signals is found during propofol-induced general anesthesia, which is similar to that of slow-wave sleep (SWS).. However, a complete understanding is lacking in terms of the characteristics of EEG changes in rats after propofol administration and whether propofol acts through natural sleep circuits.. Here, we examined the characteristics of EEG patterns induced by intraperitoneal injection of propofol in rats.

    Amplification:

    Article Title: Villification of the intestinal epithelium is driven by Foxl1 through activation of PDGFRα and BMPs.
    Article Snippet: Sections were blocked with blocking buffer (Abcam, ab126587) for 1 hour at room temperature. .. The antibody was detected using a biotinylated goat anti-guinea pig secondary antibody, followed by signal amplification using the biotin–avidin system (Vector Labs, SP-2001) and tyramide-based amplification (PerkinElmer, NEL700A001KT) according to the manufacturer’s instructions. .. The antibody was detected using a biotinylated goat anti-guinea pig secondary antibody, followed by signal amplification using the biotin–avidin system (Vector Labs, SP-2001) and tyramide-based amplification (PerkinElmer, NEL700A001KT) according to the manufacturer’s instructions.

    Avidin-Biotin Assay:

    Article Title: Modulation of striatal glutamatergic, dopaminergic and cholinergic neurotransmission pathways concomitant with motor disturbance in rats with kaolin-induced hydrocephalus
    Article Snippet: Sections were then incubated with guinea pig anti-substance P (ab10353; Abcam) antibody for 18 h at 4 °C. .. After several rinses in PBS, sections were incubated with biotinylated goat anti-guinea pig secondary antibody (BA-7000; Vector Laboratories) for 1 h, and then with avidin–biotin-complex alkaline phosphatase conjugate reagent (AK-5000; Vector Laboratories) for another hour at room temperature. ..

    other:

    Article Title: Villification of the intestinal epithelium is driven by Foxl1 through activation of PDGFRα and BMPs
    Article Snippet: Sections were blocked with blocking buffer (Abcam, ab126587) for 1 hour at room temperature.



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